Lady Rain has been making her own tempeh for years, because she knows that the freshly made kind is irresistible!
Tempeh is made from fermented soybeans. It originated from Indonesia, where soy is a staple source of protein for the people and where consumption of meat is considered a luxury. It is a popular vegan food as a substitute for meat and is gluten free.
The production of tempeh involves soaking soybeans for several hours and cooking them, before letting them ferment. Fermentation is complete within 48 hours, when the soybeans are bound together by layers of white mycelium from a species of white fungus called Rhizopus oligosporus. The solid mass of tempeh can then be cut into slices or chunks and added as an ingredient for other recipes.
This guide will show you how to make your own tempeh starter, as well as provide recipes for how to work this versatile meat substitute into your cooking.
What Is Tempeh Starter?
It is a fermentation starter for fermenting the soybeans and binding them into a cake. Basically, the tempeh starter contains spores of Rhizopus oligosporus and some rice flour—it is very easy to make. It costs almost next to nothing to make, other than paying for a packet of commercially made tempeh, plus your time and effort.
So, you will need to buy a small packet of tempeh to make more of it. It is similar to making homemade yogurt—if you want to make yogurt, you need to buy a small tub of it with live culture as a starter.
Where to Find Tempeh Starter
If you do not live in Indonesia, finding the tempeh starter can potentially be quite difficult. Although starters can be ordered online, they often come at a rather costly price. If you have never made tempeh before and just want to experiment with making it, however, you won't want to buy a packet of starter good enough to make 10 kilograms of tempeh. And you don't even know if you are going to end up with something that's edible or an epic fail!
Luckily, you can find packets of tempeh in the refrigerated or frozen section of most Asian stores and some supermarkets. I would give the supermarkets a miss, because they tend to stock marinated tempeh. We need plain tempeh to make the starter.
What to Look for in Store-Bought Tempeh
A packet of store-bought tempeh should have traces of spores that are invisible to the naked eye. A milky white tempeh will have a lower spore count as compared to one that has tiny black dots on it. So, if you are after some spores, try to choose one that looks a little "dirty" or has traces of black dots. If you can't find one with black spores, you can make the white tempeh "mature" and sporulate (grow spores).
Note: Avoid tempeh that show signs of any other colors. They are probably not fresh.
Setting Up the Incubator
If you live in a cold or cool temperate area below 25°C, an incubator is a must for making tempeh—otherwise, you will never be able to produce a successful batch. When you are ready to make the starter, you will at the very least need to improvise an incubator. Your homemade incubator must be able to maintain a temperature of 32°C. Test the incubator to see if it can provide a stable temperature for making tempeh later.
If you happen to have an old bar fridge or a big insulated container waiting to be discarded, don't throw it out just yet. It is perfect for incubating tempeh, because the thick, insulated walls minimizes heat loss (especially in winter), and it does not require more than 10W to operate.
How I Set Up My Homemade Incubator
My homemade incubator has a small 10W heating mat (for pets), a hot water bottle, and an aquarium thermometer. The heating mat rests on a rack in the centre of the bar fridge and is covered with some tea towels and another metal rack over it. This prevents direct contact of the tempeh with the heating mat, which may cause overheating and uneven heat distribution.
The hot water bottle is placed at the bottom of the fridge and the thermometer at the top of the fridge. Heat from the hot water bottle at the bottom will rise and get distributed all over the fridge, and the temperature is recorded by the thermometer near the freezer at the top of the fridge.
Approximate Fermentation Time
|Prep time||Cook time||Ready in||Yields|
36 hours 50 min
37 hours 35 min
1 slab of tempeh, 4 servings
Making the Tempeh Starter
Cut out a chunk from your store-bought tempeh and eat the rest. Hopefully, this will be the last time you have to eat commercially manufactured tempeh! The small chunk will become the starter for all your homemade tempeh later.
Place the chunk in a small bowl and cover with cling wrap. Pierce holes all over the cling wrap with a toothpick, and place the bowl in the incubator for at least two days.
Check the temperature every five hours or so to be sure it does not go below 28°C or above 35°C.
After 24 hours, you should be able to see some changes on the piece of tempeh. It will either have fluffy white mycelia growing on it, or it will have started to turn grey. If it is white and fluffy, the starter is not ready yet.
Note: If it turns slimy and smells foul, it is no good and should be discarded immediately. Start with another fresh chunk of tempeh, that is, if you haven't already eaten the remainder!
Leave it in the incubator until traces of black appear. Black means the culture has started to sporulate, producing the black spores we are after.
When there is enough grey or black stuff on the piece of tempeh, remove it from the incubator and cut it into thin slices or very small chunks. Leave the chunks to air dry for a couple of days.
Blend the dry chunks with double the amount of rice flour until they are in powder form.
This will be your starter, and it is enough to make at least 10 batches of tempeh. The homemade tempeh starter needs to be stored in an airtight container in the fridge. It can stay in the fridge for about a week. For longer storage, put it in the freezer.
In the containers below, the starter on the left was made from a fresh piece of tempeh without visible black spores. Fermentation of tempeh with this starter tends to take longer as compared to fermenting with the black spores starter in the container on the right. In conclusion, if there are black dots all over the tempeh, the spore count is definitely higher. The more black dots in the starter, the better!
How to Start Making Your Own Homemade Tempeh
At last, you can start making some tempeh. Now that you have already made your own starter, making your own homemade tempeh will be a breeze, believe me!
- 1 cup soybeans, soaked and cooked
- 1–2 tablespoons vinegar
- 0.5–1 teaspoon homemade tempeh starter
- Measure one cup of soybeans and soak them for at least eight hours or overnight. Pour away the water used for soaking and top up with clean water. Boil the soybeans for at least 30 minutes or until they are soft. Another way for softening the soybeans is to boil them for 10 minutes and then transfer them to the thermal cooker to continue cooking for another hour or so.
- Drain the water and dehull the soybeans. I find that leaving some hulls with the beans does not affect the taste or quality of the tempeh.
- Add 1 tablespoon of vinegar to the beans. Place the pot of beans over the stove and stir. This process helps to remove the excess water quicker. The beans should not be soaking wet or too dry for making into tempeh.
- Set aside the soybeans to cool to room temperature.
- Add in 0.5–1 teaspoon of the homemade tempeh starter and mix well. Transfer the beans to a glass bowl and cover with cling wrap. Prick holes 1–2 centimeters apart on the cling wrap with a toothpick. The bowl of beans is ready for incubation.
- Put the bowl in the incubator. Check the temperature every five to six hours. Some white stuff should be visible after 12 hours if the temperature is correct. Maintaining the temperature at around 32°C is very important, otherwise the tempeh will not turn out well.
A white fluffy layer of mycelium will eventually cover the beans, after around 30–36 hours. At this stage, black spores might start to appear in some areas. If not, leave the tempeh a little longer in the incubator.
The tempeh should not smell foul. If it does, discard it because it is not edible anymore. The fermentation normally does not take more than two days, and the mycelium should be distributed all over the beans.
When the soy beans are all covered with white mycelium, remove the tempeh from the incubator. It can then be used for cooking right away—freshly made tempeh is irresistible!
Please Rate This Article on Making Tempeh Starter and Fermenting Tempeh at Home
Experiment With Different Tempeh Varieties
Commercially, you might not be able to find tempeh made from other types of beans, since soybean tempeh are the most common in the market. It can also be made, however, with other legumes like chickpeas, adzuki beans, black beans, barley, and mung beans.
These beans can be made into tempeh by following the instructions above and placing them in Ziploc bags for fermenting. Always remember to pierce holes in the bags before putting them in the incubator. The bags can be stored in the freezer for many months, and you'll never run out of tempeh again!
Tips for Cooking Tempeh
- Slice and pan fry each side for a few minutes until a little brown.
- Add pan-fried tempeh to curry dishes and stews; use them in soups; simmer tempeh in tomato sauce or sweet and sour sauce; or dip them in chili sauce.
- Slices of tempeh can also be marinated and then deep fried until crispy. It makes a good snack.
- Side dishes that can be served with tempeh are white radish cakes, deep fried curry puffs, and salted duck eggs.
Tempeh is indeed a very versatile ingredient for cooking!
More Great Tempeh Recipes
- Tempeh Yakitori With Broccoli
- Vegan Tempeh Tikka Masala With Cashew Cream
- Vegan Tempeh Ragu With Spiralized Zucchini
This content is accurate and true to the best of the author’s knowledge and is not meant to substitute for formal and individualized advice from a qualified professional.
Questions & Answers
Question: Will this work if my tempeh has already been frozen?
Answer: Yes, I use frozen tempeh because that's the only one I can get.
Question: Why is it necessary to add rice flour to make tempeh? Can't we just make tempeh starter using tempeh alone? Why is it necessary to cut the tempeh starter with rice flour?
Answer: If you blend a piece of tempeh, it sticks together in a lump. Blending it with some rice flour gives you the tempeh starter in dry powder form. The idea here is to make a tempeh starter in powder form so that it can be used to sprinkle over the soybeans later.
Question: Can you make tempeh starter in an instant pot under the yogurt mode?
Answer: You can use any vessel as an incubator for making the starter, as long as a temperature of 32°C can be maintained.
© 2015 lady rain
lady rain (author) from Australia on July 31, 2018:
Linda, yes, put the chunk of tempeh and rice flour in the blender. The whole idea here is to make a tempeh starter in powder form so that it can be used to sprinkle all over the soybeans later.
linda on July 31, 2018:
Great article about making your own tempeh starter! However, could you please clarify, in your article:
"Blend the dry chunks with double amount of rice flour until they are in powder form."
Please tell me how to "blend" the dry chunks? Do you use a blender to make it into a powder, then add rice flour to it?
Thanks very much,
Andrew on April 19, 2018:
Wow! Most complete article on tempeh on the internet. Thanks so much for taking the time to put in all the detail.
lady rain (author) from Australia on October 06, 2015:
Carb Diva, I'm glad you like this hub on tempeh. I hope you'll be able to make your own tempeh one day. Thank you for the lovely feedback.
lady rain (author) from Australia on October 06, 2015:
peachpurple, tempeh is originally made from soy but you can use chickpeas, mung beans, barley and other legumes if you make your own tempehs.
Linda Lum from Washington State, USA on October 06, 2015:
Wow, this is a very impressive hub on tempeh. I had no idea that you could make your own. My younger daughter is vegetarian--we enjoy the taste of tempeh but it is quite expensive. I'm not sure I am brave enough to attempt this. Luckily, there is an Asian market not very far from our town and we can obtain good-quality tempeh whenever we want it. Thank you also for the recipes. Have a wonderful day.
peachy from Home Sweet Home on October 06, 2015:
oh now i know that tempeh is made from soy beans and vinegar. Taste delicious with chillies